Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). 316 grating motor driver faulty. With SOFTmax PRO, the contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, making it possible to. , Sunnyvale, CA). The GH Pro 340 Supplemental Greenhouse LED grow lighting is designed with greenhouse operations in mind. All absorbance measurements were taken on a SpectraMax 340 PC reader equipped with SoFTMax Pro software. Fibrin formation was measured by turbidity at 405 nm using a SpectraMax 340 PC plate reader. Samples were analyzed in triplicates. Vessel Sprout Formation Assay. Service parts for these models are not being produced and are no longer in stock. Optical density was measured at 570 nm using a microplate reader (SpectraMAX 340, Molecular Devices Co. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). The reaction mixture consists of 100 mM potassium phosphate buffer (pH 7. The Gemini EM Reader with dual monochromators allows researchers to try new and novel dyes without having toThe absorbance was determined to be 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. Hydrolysis of compounds 9-15 was monitoredAbsorption was measured at 405 nm against 490 nm using a microplate reader (SPECTRAmax™340; Molecular Devices, Palo Alto, CA, USA). ) to the upper chamber, followed by measurement with a spectrophotometer (SpectraMax 340, Molecular Devices LLC, Sunnyvale, CA, USA) at 610 nm of media in the lower chamber after one hour . Amino Acid Analysis. 2 M DEEMM were mixed in a microtube,. Driessen*,MaaikeA. using SpectraMax i3 (Molecular devices, San Jose, USA). The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). PathCheck SensorThe optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. using SpectraMax i3 (Molecular devices, San Jose, USA). 05% at 340 nm Photometric stabilization Instantaneous Photometric drift None—continuous referencing of monochromatic output Calibration Automatic before first kinetic read and. Quick View. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. The SpectraMax Vertical 1000 uses only the highest-efficacy ams-Osram LEDs with efficiencies up to 4. View the Molecular Devices website. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. MOLECULAR DEVICE M5. Temperature Range: Ambient + 4 °C up to 45 °C. SpectraMax Injector Cartridge with SmartInject™ Technology. automated SpectraMAX 340 (Molecular Devices, Sunnyvale, CA, USA) microtiter plate reader. the SpectraMax iD3 is a fantastic plate reader for a. Column chromatography was performed on silica gel (Kieselgel 60, 70–230 mesh, Merck). SpectraMax 340 Absorbance Microplate Reader, Molecular Devices (CAT#: STEM-SPO-0021-ZJF) Labsystems Fluoroskan II Fluorescent Microplate Reader, Excitation Wavelength Range 320 – 700 nm, Labsystems (CAT#: STEM-SPO-0006-LGZ) BioTek MQX200 Microplate Reader Spectrophotometer, 24VDC, BioTek Instruments, Inc (CAT#: STEM. 5 mL sample or standard solution was well mixed with 0. Bioz Stars score: 86/100, based on 1 PubMed citations. SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Molecular Devices SpectraMax Gemini XS Microplate Reader. The activity was measured in a SpectraMAX 340 plate reader at 37°C and 410 nmol/l for 120 sec. These isotypes cause antigen cross-linking by multimeric binding and self. LAL Reagent Grade ™ Multi-well Plates (Lonza cat. Each experiment was performed in duplicate and repeated at. Percent radical scavenging activity was determined by comparison with a DMSO containing control (shown in Table 3). This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536. Filipin StainingSpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro data analysis software. The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurements in the visible range. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. 303 unable to cal dark current. Lactate dehydrogenase (LDH) release assay Cytotoxicity was determined by measuring the release of LDH. The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. 2. Unit has several areas of damaged plastic. #ABS PLUS) Methods. The reduction of HED in the presence of NADPH and GR was recorded at 340 nm. 340 nm. Cell viability wasDescription Microplates The SpectraMax Multi-Mode Microplate Reader can accommodate SBS- standard 6-well to 384-well microplates and strip wells. 340-850 nm: Wavelength Selection: Monochromator, turnable in 1 nm increments:. TGFα release was calculated as described in the Results. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536. Tailored to your different needs, these kits are configured and optimized for Molecular Devices SpectraMax® microplate readers with preconfigured protocols provid- plates can be used in the SPECTRAmax PLUS 384. The results of ELISA optical density (OD) values in duplicates were averaged for the following analyses. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. . Recalcification (clotting) time was determined by monitoring absorbance at 650 nm at 10-sec intervals in a SpectraMax 340 microtiter plate reader, with onset time (the time to a linear increase in the OD, which reflects the maximal rate of formation of insoluble fibrin) set at an OD of 0. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. 00 USD. This test was performed in duplicates. Narrow bandwidths deliver increased measurement accuracy and linearity over a wide range of assays. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. Spectrophotometer (SpectraMax 340). 00 USD. Details. Although all samples initially were analyzed at the same volume, the volume of sample. Cells were scraped from the plate and single cell suspensions were made by passing cells through 28G1/2 insulin syringes. N-34-LB. This was followed by an additional incubation at 37 °C for 30–60 min. Methods: We investigated growth inhibition by varying concentrations of AMB in seven isolates of C. 30 µs. 1 in stock! Molecular Devices SpectraMax M2 Multilabel Microplate Reader. Includes: SpectraMax 340PC384. TDP-43 stress granular cells were treated with 70% ice-cold. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. Introduction The SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro Data Acquisition and Analysis Software. Introduction The SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature. Details. 4; 100 m M NaCl, 0. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The ratio of red to green fluorescence intensity was calculated. 340/80 nm excitation with 616/10 nm emission range Optimized for use with ScanLater Western Blot Detection cartridge for the SpectraMax® i3, i3x, and Paradigm® Multi-Mode Readers Optimized for use with the enhanced TRF detection module for the SpectraMax iD5 Multi-Mode Microplate ReaderThe optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance. The. Briefly, 50 µl-samples were incubated with equal volumes of Griess reagent (1% sulphanilamide, 0. 7. The release of p-nitrophenol ( 400) 17000 M-1 cm-1) was monitored at 25 °C for the enzymatic hydrolysis of com-pounds 1-8. A microplate reader asThe absorbance was measured at 425 nm and read every 10 min using a Spectramax 340 plate reader (Molecular Devices ®, USA). This Molecular Devices SPECTRAmax 340PC incorporates a holographic grating. SpectraMax Multi-Mode Microplate Readers. Create custom fluorescence assays without having to purchase expensive filter sets. Microplate Readers. A 0. Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). edu Contact Phone: 216-368-8962. About The SpectraMax GEMINI EM is controlled by an external computer running SoftMax ® Pro software which provides integrated instrument control, data display, and statistical data analysis. Description Control Panel Cuvette Chamber Back Panel Microplate Drawer The Control Panel TEMP on/off TEMP Figure 1. Awareness Technology, Inc. The VersaMax and SpectraMax 190 read 96-well microplates. 30. Alkaline phosphatase (ALP) activity. As discussed below, dilution of the culture was required because cell density is not proportional to A 600 when the values are above ;0. 5mM), and changes in OD 405 nm. 8–250 nM) was measured as follows: the prothrombinase reaction proceeded for 3 min and was stopped by the addition of 10 mM EDTA. 5 stars - 1602 reviewsThis assay typically develops in 1. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. IMAP assays are based on binding of phosphate to immobilized metal coordination complexes on nanoparticles. 5 ng/ml; estradiol: 10 pg/ml. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. Incubation with TMB substrate was done for 18 min. Features showcased by this model include. Amino Acid Analysis. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. D2. The absorbance at 405 nm was determined using a microplate reader (spectraMAX 340). 2 Chemical Reagents 1. Rate of fibrinolysis. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. Exhibit with us {{>currencyLabel}} Back. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. An absorbance was read at 490/630 nm using a spectraMAX 340 microtiter plate reader. 5. Lightgreenglass,2 mmthick(340 nm) 9:Yellowglass,3 mmthick(405 nm) 10:Darkredglass,3 mmthick(650 nm)automated SpectraMAX 340 microtiter plate reader. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. 2 0. For use with Spectramax 340 devices which have an 8-pin mini DIN serial socket. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). #N207) LAL Reagent Water (Lonza cat. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. 0% and ± 0. Alkaline phosphatase (ALP) staining assay ALP activity was detected using a SensoLyteVR pNPP Alkaline Phosphatase Assay Kit (AnaSpec, Inc. 2. Molecular Devices Spectramax 340 Absorbance Plate Reader (1997) Molecular Devices SpectraMax i3x Multi-Mode Microplate Reader (2016) Vernier UV-VIS Spectrophotometer (5) Vernier VIS Spectrophotometer (30) Infrared Spectroscopy. Operators can conveniently select up to 6 wavelengths at a time for. The optical density of each sample was analyzed at 450 nm with a reference reading at 630 nm using a SpectraMax 340 absorbance platereader (Molecular Devices, Union City, CA, United States). For 40 years, we have partnered with scientists to help them achieve landmark discoveries. Availablility: By Availability Molecular Devices SpectraMAX 340 Microplate Reader. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Measurements of OD at 540 nm were then performed in a microtiter plate reader (SpectraMax 340 microplate reader; Molecular Devices Corp. These lab workhorses are used in drug discovery, research, bioassay validation, and. Oxidative stress induction by H 2 O. ATI Note: #1: The N-34-MLA is a replacement assembly which replaces an OEM lamp. Each experiment was performed in duplicate. 315 can't find zero order. The results are expressed as the percentage inhibition of BrdU incorporation compared to the untreated control. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. The SpectraTest validation package provides a NIST-traceable solution for validating optical performance of the SpectraMax Plus 384 reader and does it automatically. Absorbance was read at 405 nm on a microplate reader (SPECTRAmax 340 PC). Photometric Performance SpectraMax 340PC384 Item Description Wavelength range 340 nm to 850 nm Wavelength selection Monochromator tunable in 1 nm increments Wavelength bandwidth. Call 1-800-745-2710. Filterless readers provide consistent results with steady temperature regulation. Details. 7. The cell concentration was measured by a spectrometer (SpectraMax 340, Molecular Devices, USA) with an optical density at 600 nm (OD 600). The SpectraMax ABS Plus, can accommodate standard 96-well plates and 384-well plates. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. SpectraMax ® Plus 384 optics. The increase in fluorescence was determined for 20 min by excitation at 520 nm and emission at 545 nm in a. 340 to 740 nm (linear variable dichroic) 220 to 1000 nm for abs (spectrometer) Plate Formats: 6 to 384-well plates, LVis Plate with sixteen 2 µL microspots. The relative LDH release was determined as the percentage ratio of the optical densities in the media (containing CNMs at each. SpectraMax 190 reads 96-well and strip-well plates. This Molecular Devices SPECTRAmax 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. #N207) LAL Reagent Water (Lonza cat. Includes: Computer Loaded with Softmax Pro 5. The SpectraMax i3x reader provides the flexibility to add novel detection capabilities without the need for service engineers or costly downtime—it grows with you as your research areas expand. The fluorescence intensity was measured using the SpectraMax 340 microplate reader (Molecular Devices) at the following conditions: excitation at 475 nm and emission at 529 nm (green fluorescence) or 590 nm (red fluorescence). Apoptosis was also measured by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) assay using the TACS Blue label kit (4811-30-K, R&D Systems). When you do reads at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. The SPECTRAmax 340PC can mea- TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytes acrossawiderangeofconcentrations. #ABS PLUS) SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. Background: A computerized machine, SPECTRAmax 340, was used to evaluate the recently reported phenomenon of antagonism of the polyene amphotericin B (AMB) in Candida albicans pre-exposed to the triazole fluconazole (FLZ). This is a used Molecular Devices SpectraMax 340PC Microplate Spectrophotometer. Like CD3, the CD95 death receptor requires binding of immobilized or multimeric antibodies to be activated effectively. The maximum specific growth rate μ was calculated from the exponential growth phase ( 52 ) and plotted against the concentration of the different drugs. To screen for antigen specific IgG, 50 μL/well of biotinylated antigen at a concentration of 20-50 ng/mL was added and incubated for one hour at RT. With SOFTmax PRO, the contents of the wells in a microplate can be mixed used in the SPECTRAmax PLUS. 2. ). When you do a read at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. Please refer to photos for further. Time-resolved fluorescence was measured on the SpectraMax i3x and SpectraMax iD5 readers using preconfigured protocols in SoftMax® Pro Software (see Tables 2 and 3 for instrument-specific settings). Samples with OD values of less than 0. 5. Each experiment was performed in duplicate and repeated at. Confocal laser scanning microscopy (CLSM) Biofilms were formed on glass-bottomed Petri dishes (CELLview™; Greiner Bio-One GmbH, Frickenhausen, Germany) in the presence or absence (control biofilms) of the BIC 50 of antifungals as described above. These robust readers have been placed in labs from Antarctica to the. The assays contained 0. Streamline GLP/GMP compliance. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. The spectrophotometers used were SpectraMax 340 (for GSH, GCS, and HNE-His assays) and SpectraMax M3 (for GGT, GPx, and GST assays), both from Molecular Devices, Sunnyvale, CA. VersaMax reads 96-well and strip-well plates. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Not all manufacturers' microplates are the same with regard to design, materials, or76358-624. WST regents of 10% were added to each well, and absorbance was determined at 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. The optical density of each well was determined immediately using a microplate reader (SpectraMax-340, Molecular Devices) set to 450 nm. SpectraMax® M2/M2e user guide a multi-detection microplate reader with two-mode cuvette port SpectraMax M2e user guide cover 1 1 4/21/06 9:53:28 AM. 1% N-1-naphtylethylenediamine dihydrochloride, 2. ” SpectraMax® Quant™ dsDNA Assay Kits The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. Awareness Technology, Inc. Figure 1. MSD read buffer and TMB one component HRP substrate are from MSD and SurModics (Eden Prairile, MN), respectively. Proteolytic activity was determined using the synthetic chromogenic substrate Nα-benzoyl-DL-arginine p-nitroanilide (DL-BApNA), in 96-well plates, according to Ponce-Soto et al. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid- Absorbance at 405 nm (Abs 405) was obtained for both plates before (background) and after 1-h incubation at 37°C using a microplate reader (SpectraMax 340 PC384, Molecular Devices). Intra- and inter-assay coefficients of variation were lower than 8. 5. 1% N-1-naphtylethylenediamine dihydrochloride, 2. The cells were pretreated, serum starved for 24 h, and then treated with NK-cdM priora SpectraMAX 340VR microplate reader (Molecular Devices, Sunnyvale, CA, USA). The relative cell viability was determined as the percentage of the absorbance value in the cells to the absorbance value of a control group. gp70 blocking. The possi-ble effect of zinc or copper on the binding of heme by A was tested as described above by using a 50 and 100 M final concentration of each metal. The optical density at 405 nm (OD 405) of the plates was measured with a microplate reader (SpectraMax 340 PC384, Molecular Devices) before and after a 1-h incubation at room temperature. SPECTRAmax® 190. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. Glutathione reductase (GR) activity was measured, according to Smith et al. 5. With SOFTmax PRO, the contents of the wells in a microplate can be mixed SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. Figure 1. The assay was carried out in a 96-well plate in a final volume of 100 µl containing 50 mmol/l Tris-HCl, pH 7. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Molecular Devices Spectramax 340 Plate Reader Repair. SpectraMax Multi-Mode Microplate Readers. (Shiga, Japan) and kept for 30 min in the dark. using SpectraMax i3 (Molecular devices, San Jose, USA). chen@case. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. Wavelength(s) 340 to 850 nm; Add to Compare List. 1 mg mL −1 CDDP loaded A-CHAM, B-CHAM, C-CHAM or D-CHAM. Isolation of RNA and RT-PCR. Call 1-800-745-2710. Media in plates were withdrawn by pipetting and 200 μl of DMSO was added to each well to solubilize the formazan crystals. The light features a slim profile to prevent. Rapid, accurate DNA, RNA, and protein quantitation in a one-touch, full-spectrum micro-volume absorbance reader. Lactate Dehydrogenase (LDH) Release. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. Analysis of ALA by Ehrlich’s Reagent Assay. The supernatants were mixed with an LDH assay reagent, which was purchased from Takara Bio Inc. Not all manufacturers' microplates are the same with regard to design, materials, orAbsorbance at 405 nm (Abs 405) was obtained for both plates before (background) and after 1-h incubation at 37°C using a microplate reader (SpectraMax 340 PC384, Molecular Devices). 304 signal level saturation. 190-1000 nm, tunable in 1 nm increments. , Oxon, UK). California Lightworks uses only the highest quality LEDs from Osram. 340/80 nm excitation with 616/10 nm emission range Optimized for use with ScanLater Western Blot Detection cartridge for the SpectraMax® i3, i3x, and Paradigm® Multi-Mode Readers Optimized for use with the. LDH release was measured with a commercial kit following the manufacturer’s instructions (LDH Cytotoxicity Detection Kit, TaKaRa). Laser Scanning Confocal Microscopy The fluorescent signals were obtained using a Zeiss LSM 510 laser scanning confocal microscope. Availablility: By Availability Molecular Devices SpectraMAX 340 Microplate Reader. the SpectraMax iD3 is a fantastic plate reader for a wide range of assays. Quick View. A 0. Microplate Spectrophotometers. Here, we show how the SpectraMax® i3x and SpectraMax® iD5 Multi-Mode Microplate Readers are used to perform robust, no-wash saturation binding assays using HTRF. TheSpectraMax®Plus384addstheabilitytoreadcuvettes. Sandwich ELISAs were used to measure insulin (DY8056, R&D Systems, Minneapolis, MN, USA). 2. Acceptor: 665nm. PC12 or BV-2 cells treated with various concentrations of BBD were stressed with hypoxia for one hour and the supernatant was then assayed for LDH activity. 5. 0 2. Acceptor filter: 665 nm. PGD2 concentrations in samples from treated cell cultures were determined using the linear range of a standard curve and are presented as percentages of the PGD2 levels measured in the solvent controls. 190–850 nm <± 1. Continuous kinetic monitoring of enzyme activity was performed on SPECTRAmax 340 or Flexstation 2-384 microplate reader (Molecular Devices, Sunnyvale, CA, USA) and controlled by the Softmax software. 5 mm (bottom read) Beam diverges to focal spot near bottom of well. ELISA plates were read with a Spectramax 340 from Molecular Devices (Sunnyvale, CA, USA). Molecular Devices SpectraMax 384 Plus Microplate Reader Specifications. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. edu Contact Phone: 216-368-8962. Call 1-800-745-2710. Created Date: 20051213090753Zmeasured using a SpectraMax 340 multiwell plate. The narrow bandwidth used by the model increases measurement accuracy and broad analysis over. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. Hydrogen peroxide (H 2 O 2) was used to establish an intracellular oxidative. Finally, the absorbance at 450 nM was taken using a SpectraMax ® 340 ELISA plate reader (Molecular Devices, USA). Excitation 1 340 nm Emission 1 520 nm Cut off for emission 1 495 nm Excitation 2 340 nm Emission 2 620 nm Cut off for emission 2 570 nm Sensitivity Readings 50 to 100 PMT Optimal Column Wavelenght Column Priority . The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Microplate Readers. 2. 8 pin mini DIN (Spectramax 340) to 9D (PC) serial data transfer cable. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. SPECTRAmax® 190. Labsystems Luminoskan RT Microplate Reader. SpectraMAX 340 plate reader (Molecular Devices). 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideHigh-throughput assays for promiscuous inhibitors Brian Y Feng1,3, Anang Shelat1,3, Thompson N Doman2,RKipGuy1 & Brian K Shoichet1 High-throughput screening (HTS) searches large libraries of chemical compounds. ThermoMax performs automatic mixing before and between readings to ensure reliable and precise. 9000-0756. 0, 20 mmol L –1cal-cium chloride). Developed plates were read on MSD reader Sector Imager S 600, Model 1200, with Discovery Workbench Software (version 4. The screening was initiated by adding 10 μL p-nitrophenyl phosphate (pNPP), and the catalysis of pNPP (1 mM) was continuously monitored on SpectraMax 340 microplate reader (Molecular Devices, Sunnyvale, CA, USA) at 405 nm for 20 min at 37 °C. Examples: “Press carefully to engage,” “Do not press down. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening SoftMax® Pro 7. Molecular Devices Spectramax 340PC instructions. 4) was followed according to Vanacker et al. The reaction mixture was incubated for 5 min. After adding the stop solution, the absorbance at 450 nm with a wavelength correction at 540 nm was measured with a SpectraMax 340 plate reader (Molecular Devices, San. View the Molecular Devices website. SpectraMax Multi-Mode Microplate Readers. 2. Samples were plated in duplicate (r=0. 2. 5), L-ascorbic acid (25. For cell permeabilization, 1 ml of Z buffer (60 mM Na 2 HPO 4 z 7H 2 O, 40 mM NaH 2 PO 4 z H 2 O, 10 mM KCl. 7 macrophages were obtained from ATCC (Manassas, VA). Absorbance was read at 405 nm on a microplate reader (SPECTRAmax 340 PC). . SOD activities were calculated from assays in which there was an inhibition of cytochrome c reduction of between 40 and 50%. Since the formation of NADPH is stoichiometrically related to the oxidation of glucose-6-phosphate, measurements for glucose + fructose can be determined by the absorbance of NADPH at 340 nm. One plate carrier adapter is provided with the instrument. edu Contact Phone: 216-368-8962. , Sunnyvale, CA). 00. Or scan up to 384 wells at any time to determine the best assay conditions. With a wide range of on-board features and capabilities, thermoax is ideally suited for use in modern laboratories involved in many applications. Briefly, hPDLSCs were cultured in osteogenic/odontogenic induction medium for 14. Call 1-800-745-2710. ScanLater Western Blot Detection Cartridge. CD154 is an established indicator of antigen-reactive T helper cells in other species, but has not been characterized in horses. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 1 mL of culture media with 10% FBS. When necessary, samples were. . The manufacturer of the SpectraMax 340PC Microplate Spectrophotometer is Molecular Devices. 85%. Statistical analysis The significant differences between the different strains in the adherence assays were analyzed using the Student’s t -test. Plate reader (SpectraMax 340 PC plate reader or comparable plate reader). When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the. SpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro data analysis software. The absorbance is determined at 620 nm on a SpectraMax 340 PC 384. The SpectraMax® iD3 Multi Mode Microplate . 340/70 nm. SpectraMax 340PC384 reads 96-well plates and 384-well plates and strip-well plates. , Fremont, CA, USA). The values of IC 50 represent concentrations of compounds toA microplate reader (SpectraMax M5; Molecular Devices, Sunnyvale, CA) was used to measure the absorbance at 340 nm for the collected BPW (6) Stabilization protects islet integrity during respirometry in the Oroboros Oxygraph-2K analyzer Islets December 31, 2022 Justin J. Our absorbance plate readers feature our PathCheck Sensor technology and. Data were expressed as the mean percent viable cell vs. Western blot. Narrow bandwidths deliver increased measurement accuracy and linearity over a wide range of. SpectraMax Multi-Mode Microplate Readers. 3. Features demonstrated by the model include temperature control, a robot-compatible interface and data analysis software. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. EX range: 340/80 nm EM range: 616/10 nm High fg levels of Streptavidin High fg levels of Streptavidin 2 AlphaScreen. Following cell culture under normoxic or hypoxic con-ditions, culture medium samples were obtained on completing the incubation period to measure atrial natriuretic peptide. #N207) LAL Reagent Water (Lonza cat. Absorbance was read at 450 nm using a Molecular Device Spectramax 340. This reader only allows high performance HTRF measurement when assays are run in. Not all manufacturers' microplates are the same with regard to design, materials, orIn a typical 100-μL assay with mixtures containing 50 mM MOPS, pH 6. Capable of detecting DNA down to 25 ng per well, this model gives users the same sensitivity as conventional systems to produce accurate nucleic acid measurements. Water – distilled water or equivalent. 0 nm increments Wavelength Bandwidth: 2 nm Wavelength Accuracy: < ± 1. LAL Reagent Grade ™ Multi-well Plates (Lonza cat. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. The contents of the. The cell growth was determined by measuring the optical density at 600 nm using a spectrophotometer (SpectraMax 340, Molecular Devices, USA). Molecular Devices ThermoMax is a high-efficiency, temperature-regulated, 96-well microplate reader. Molecular Devices’ PathCheck® sensor technology. Or scan up to 384 wells at any time to determine the best assay conditions. The optical density (OD) at the wavelength of 450 nm was measured with a spectrometer (SpectraMax 340, Sunnyvale, CA, USA). 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. Near bottom. Wavelength range: 340-850 nm. 5. Bertin Precellys Evolution Touch Homogenizer. SpectraMax i3x 和 SpectraMax iD5 微孔板读板机上使用 SoftMax Pro 软件中的预设方法进行测量(有关仪器特定设 置，请参见表 2 和表 3 )。在 SpectraMax i3x 和 SpectraMax iD5 读板机上进行了微孔板优化和读取高度调整，以确保最佳 的检测灵敏度和动态范围。 数据分析SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. The concentration of NO in the cells was determined by measuring the amount of nitrite, a relatively stable oxidation product of. SpectraMax Plus 384 measuring instruments pdf manual download. No power cord or accessories included.